Figure 2.
Histochemical visualization of reporter-enzyme activity in transgenic retinas. Retinas were doubly stained with X-Gal and X-Phos (A–F, H) or with X-Gal and naphthol phosphate/fast red (G) to reveal lacZ-expressing cells (blue) and AP-expressing cells (purple/brown or red), respectively. Flat mounts: (A) pPMS107 1E2; (B) pPMS114 1D5. Vertical sections: (C) pPMS107 1E2; (D) pPMS108 1H11; (E) pPMS114 1D5; (F) pPMS101 1E12; (G) pPMS61 2B3; (H) pPMS101 1E12, showing a rare AP-only cone in a lacZ≫AP retina. All retinas except for that shown in G are from floxed mice.
