Table 1.
Numbers of transgene-expressing cells (AP-only, lacZ-only, AP+lacZ)
| Construct | ES line | Chimera | Nonfloxed | Floxed |
|---|---|---|---|---|
| pPMS107 | 1E2 | 707, 161, 114 | 798, 1292, 1105 | |
| 2D4 | 590, 167, 1191 | |||
| 2F4 | 517, 62, 27 | 1263, 539, 534 | ||
| pPMS108 | 1H11 | 4402, 34, 107 | 4447, 68, 551 | |
| 2A12 | 0, 0, 0 | |||
| pPMS101 | 1E12 | 0, 2386, 0* | 2, 2715, 0* | |
| 1G8 | 1, 2382, 0 | |||
| 2C3 | 0, 947, 0 | |||
| 2D11 | 2, 930, 0 | 5, 1030, 0 | ||
| 2E10 | 0, 0, 0 | |||
| 2G10 | 0, 461, 0 | |||
| 3A6 | 8, 2605, 0 | |||
| pPMS114 | 1B6 | 1479, 46, 525 | ||
| 1D5 | 189, 30, 812 | 173, 4, 1018 | ||
| pPMS61 | 2A1 | 321, 1, 2620 | ||
| 2B3 | 534, 2, 8136 | |||
| 2B11 | 352, 22, 5160 | |||
| 2C11 | 77, 2, 401 | |||
| 2E7 | 427, 3, 3288 | |||
| 2E11 | 1041, 273, 4503 |
*
For line pPMS101 1E12, the central number indicates lacZ≫AP. In this line, >99% of expressing cones show intense lacZ activity and extremely low levels of AP activity. Faint AP staining is detectable only in the outer segments, a region of high-membrane content that is largely devoid of lacZ activity (Fig. 2H).