Table 2.
Fold changes in KM for ATP, A0.5 for 3-PGA activation, and I0.5 for Pi inhibition for the various Devo mutants as compared to TG-15
| Class | Strain | Mutations | ATP
|
3-PGA
|
Pi
|
|---|---|---|---|---|---|
| fold ↓ in KM | fold ↓ in A0.5 | fold ↓ in I0.5 | |||
| I | Devo 330 | Y315C, L378S | 2.4 | 11.7 | 18.8 |
| Devo 355 | K295E, Y315C | 1.5 | 2.5 | 5.8 | |
| II | Devo 301 | T426I | 3.0 | 1.2 | 5.0 |
| Devo 303 | K295E, L378S | 6.0 | 1.6 | 5.0 | |
| Devo 316 | L378S | 1.5 | 1.5 | 2.5 | |
| Devo 350 | K295E | 3.0 | 1.4 | 2.0 | |
| III | Devo 202 | S2P, K95R | 2.0 | 3.8 | 1.0 |
| Devo 206 | K341M | 2.4 | 2.1 | 1.0 | |
| Devo 339 | Y315C | 2.4 | 6.7 | 1.0 | |
| IV | Devo 201 | K377E | 3.0 | 1.1 | 1.0 |