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. 2002 Jun 27;2:14. doi: 10.1186/1471-2210-2-14

Figure 3.

Figure 3

Butaprost free acid mediated stimulation of intracellular cAMP production in COS-1 cells transfected with the rabbit EP2 receptor. Cells were incubated with various concentrations of PGE2 in the presence of IBMX for 5 minutes. The reactions were stopped with 10% TCA, and cell lysates were analyzed for cAMP content by a cAMP EIA kit. The data shown are from a single experiment and are representative of 3 independent experiments. ▲ rabbit EP2 transfected; ■ vector only transfected.