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. 2002 Jul 8;3:18. doi: 10.1186/1471-2164-3-18

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Copyright ©2002 Garbarino and Gibbons; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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Light microscopy of immunostained yeast cellsA: cells in interphase and during division; B, C, selected cells in which the region staining for midasin is larger (arrows) than the region staining for DNA. "Comet tails" are conspicuous in several of these cells, with short tails remaining post-division into early interphase. All fields show haploid yeast containing the MDN1(HA) gene double-stained with HA-antibody and DAPI. D, differential interference images; M, localization of HA-tagged midasin; P, localization of DNA, as stained by DAPI. All antibody-stained and DAPI fields are matched levels taken from through-focal series with steps of 0.3 μm.