Table 1.
Amino Acid Conservation in Midasin Domains
| Domain | Yeast vs Human | Giardia vs Human | Encephalitozoon vs Human |
|---|---|---|---|
| (percent) | (percent) | (percent) | |
| N-terminal | 19–38 (14) | NA | NA |
| AAA (overall) | 41–57 (9) | 31–47 (10) | 27–42 (23) |
| AAA1 | 30–45 (10) | 29–49 (19) | 26–48 (29) |
| AAA2 | 43–61 (5) | 33–50 (10) | 28–50 (18) |
| AAA3 | 65–78 (0) | 50–68 (4) | 50–67 (4) |
| AAA4 | 53–67 (23) | 41–62 (12) | 47–65 (13) |
| AAA5 | 58–71 (1) | 49–67 (2) | 34–58 (8) |
| AAA6 | 33–51 (6) | 29–47 (12) | 27–45 (36) |
| Linker | 18–34 (30) | 17–33 (31) | 28–61 (57) |
| D/E-rich | 25–45 (11) | 18–39 (5) | 28–50 (58) |
| M-domain | 45–65 (3) | 32–53 (5) | 32–53 (25) |
| Overall | 29–46 (18) | 26–44 (14) | 18–29 (47) |
The first and second numbers in each cell give the percentages of identical and similar amino acids, respectively; the number in parenthesis gives the percentage of gaps in the alignment. All percentages were determined in pairwise alignments of the indicated midasin domain, as generated by the "Blast 2 Sequences" server at http://www.ncbi.nlm.nih.gov/blast/bl2seq/bl2.html with default parameters. In cases where the domains differed in length, identical and similar are calculated as percentages of the smaller domain; gap is calculated as a percentage of the larger domain. When necessary, dummy anchor sequences were added to NH2-termini and COOH-termini in order to force the server to align full length domains.