Figure 5.

Reconstitution time (a) and reduction ratio (b) of lyophilized formulations (F1, 150 mg/ml protein concentration) with 225 mg protein in 2 ml vial, 450 mg in 8 ml vial, and 900 mg in 20 ml vial at headspace vacuum range from 250 torr to 0.1 torr. N = 10 for each analysis. Error bars represent one standard deviation. Statistically significant differences between the 250 torr, 100 torr, 50 torr, 10 torr, and 0.1 torr cakes are denoted by ^*(P < 0.05), ^**(P < 0.01) and ^***(P < 0.001). Reproduced from [82].