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. 2025 Jan 7;14:1516165. doi: 10.3389/fonc.2024.1516165

Table 2.

Known REV7 interactors.

Protein Function Organism Detection Method Reference
53BP1 DDR, NHEJ H MS, CoIP (221)
911 Complex DDR Y Y2H, P, CoIP (222)
ADP Adenovirus infection, cell lysis H Y2H, P, CoIP (132)
CDC20 APC/C, cell cycle regulation H Y2H, P, Co-IP (82, 138, 223)
CDC27 APC/C, cell cycle regulation H P, CoIP (223)
CDH1 APC/C, cell cycle regulation H P, CoIP (5, 83, 84)
CDK1 Cell cycle regulation, PGC M CoIP (105)
CAMP/CHAMP1 Cell cycle regulation, chromosome segregation H CoIP, MS, XRD (15, 90, 224)
CLTA Cell cycle regulation, chromosome segregation H Y2H, P, CoIP (91, 225)
CST-Complex DDR, NHEJ H Y2H, CoIP (69)
DDK TLS Y CoIP (226)
ELK-1 Transcription factor H Y2H, P, CoIP (19)
EspF E. coli virulence effector EC Y2H (139)
G9A Epigenetics, histone methyltransferase M CoIP (105)
GLP Epigenetics, histone methyltransferase M CoIP (105)
HCCA2 Transcriptional coactivator H Y2H, P, CoIP (227)
HR23B DDR, NER H MS, CoIP (228)
IpaB Cell cycle disruption by Shigella H Y2H, P, CoIP, XRD (16, 138)
MAD2 SAC, mitosis H Y2H, P, XRD (4, 14)
MDC9/ADAM9 Mitosis H Y2H, P (229)
NCOA3 Transcriptional coactivator H CoIP, MS (165)
p31(comet) Cell cycle regulation, SAC H Y2H, CoIP, XRD (14, 22)
p53 DDR, DSB H P (230)
POLD2/Pol31 TLS, Pol ζ Y, H Cryo-EM (17)
POLD3/Pol32 TLS, Pol ζ Y, H P, Cryo-EM (17, 231)
PRCC Cell cycle regulation H Y2H, FRET, CoIP (232)
PRDX2 Antioxidant, carcinogenesis H CoIP, MS (175)
RAN Cell cycle regulation H Y2H, P, CoIP, XRD (16, 233)
REV1 TLS Y, M, H Y2H, P, CoIP, Cryo-EM, XRD (44, 234239)
REV3 TLS, Pol ζ Y, M, H Y2H, P, CoIP, Cryo-EM, XRD (14, 235237, 240, 241)
REV7 TLS, Pol ζ, DSB Y, M, H Y2H, CoIP, NMR (14, 68, 242)
SHLD1 DSB, NHEJ M, H Y2H, P, Cryo-EM, MS, XRD (66, 70, 73, 243245)
SHLD2 DSB, NHEJ M, H Y2H, P, Cryo-EM, MS, XRD (66, 70, 73, 244247)
SHLD3 DSB, NHEJ M, H Y2H, CoIP, Cryo-EM, XRD (73, 242, 246, 248, 249)
SIM2 Transcription factor R, H Y2H, CoIP (225)
TCF4 Transcription factor H Y2H, P, CoIP (18, 250)
TFII-I Transcription factor H P, CoIP, MS (251)
TRIP13 Cell cycle regulation, chromosome segregation, SAC H CoIP, MS, Cryo-EM, XRD (21, 22, 68, 246)

REV7 interacts with proteins that perform a wide variety of functions. DDR, DNA damage response; NHEJ, non-homologous end joining; APC/C, anaphase-promoting complex/cyclosome; TLS, translesion synthesis; SAC, spindle assembly checkpoint; DSB, double-strand break; PGC, primordial germ cell. Interactions detected via co-immunoprecipitation (CoIP), fluorescence resonance energy transfer (FRET), GST-tagged pull-down (P), mass spectrometry (MS), X-ray diffraction (XRD), chromatin immunoprecipitation (ChIP), and/or yeast two-hybrid (Y2H). Organisms studied include human (H), mouse (M), rat (R), yeast (Y), and E. coli (EC).