Table 5.
Primer sequences of KASP markers developed from SNP markers significantly associated with eight QTL for high-temperature adult-plant resistance to stripe rust.
| QTL name | Physical position (Mb) a | KASP marker | Primer sequence (5′–3′) |
|---|---|---|---|
| QYrWW.wgp-1A.1 | 472.17 | IWA2995-A | GAAGGTGACCAAGTTCATGCTCATCTCCACAGTTGCAATGGT |
| IWA2995-B | GAAGGTCGGAGTCAACGGATTCATCTCCACAGTTGCAATGGC | ||
| IWA2995-C | CTGAGCAGCTGCCCTTACTT | ||
| QYrWW.wgp-1A.2 | 518.01 | IWA6710-A | GAAGGTGACCAAGTTCATGCTAATCTTGCCATTCTTTAGGGGTA |
| IWA6710-B | GAAGGTCGGAGTCAACGGATTAATCTTGCCATTCTTTAGGGGTC | ||
| IWA6710-C | GAAGATACCATTAGTGAAGCAGAAC | ||
| QYrWW.wgp-1D | 32.54 | IWA362-A | GAAGGTGACCAAGTTCATGCTGGAGTATTGCGATGAGGTGAAT |
| IWA362-B | GAAGGTCGGAGTCAACGGATTGGAGTATTGCGATGAGGTGAAC | ||
| IWA362-C | AGGAGTCTCAGGTATTGATTGATAC | ||
| QYrWW.wgp-1D | 47.85 | IWB10919-A | GAAGGTGACCAAGTTCATGCTCCTCTTGTGACTTGTGTGGGA |
| IWB10919-B | GAAGGTCGGAGTCAACGGATTCCTCTTGTGACTTGTGTGGGG | ||
| IWB10919-C | TACACCAACTGATCGAGCTAC | ||
| QYrWW.wgp-2B | 69.65 | IWB72086-A | GAAGGTGACCAAGTTCATGCTGCAGGAAAAATTGCGAGCCA |
| IWB72086-B | GAAGGTCGGAGTCAACGGATTGCAGGAAAAATTGCGAGCCG | ||
| IWB72086-C | TCCAACCGCCAAGCTTTTTG | ||
| QYrWW.wgp-3B | 66.58 | IWB5332-A | GAAGGTGACCAAGTTCATGCTACCATCATAAGCTCATCGGAAT |
| IWB5332-B | GAAGGTCGGAGTCAACGGATTACCATCATAAGCTCATCGGAAC | ||
| IWB5332-C | GGAACTGACCTGCTTGTCGA | ||
| QYrWW.wgp-3B | 66.87 | IWB10937-A | GAAGGTGACCAAGTTCATGCTTTAGTGTCAGGATGTAGATTGCATA |
| IWB10937-B | GAAGGTCGGAGTCAACGGATTTTAGTGTCAGGATGTAGATTGCATG | ||
| IWB10937-C | TCTTTCCTTCTCACTGCTTGTCA | ||
| QYrWW.wgp-4A | 700.42 | IWB39715-A | GAAGGTGACCAAGTTCATGCTAAACCGCTTTCTGGAAGAGT |
| IWB39715-B | GAAGGTCGGAGTCAACGGATTAAACCGCTTTCTGGAAGAGC | ||
| IWB39715-C | CGCCGCCGCTAATTTACAA | ||
| QYrWW.wgp-6A.3 | 447.83 | IWB22698-A | GAAGGTGACCAAGTTCATGCTAGGTGAATTGAGCTGATTGTTGA |
| IWB22698-B | GAAGGTCGGAGTCAACGGATTAGGTGAATTGAGCTGATTGTTGC | ||
| IWB22698-C | ACACGCTGATAACCACGAATAGA | ||
| QYrWW.wgp-6D.2 | 178.68 | IWA3624-A | GAAGGTGACCAAGTTCATGCTCAATATTCACAACCCCACATGTGCA |
| IWA3624-B | GAAGGTCGGAGTCAACGGATTCAATATTCACAACCCCACATGTGCC | ||
| IWA3624-C | AGAGTGCTCAGTAGGCAATCGAC |
a
The physical positions were referred to the “Chinese Spring” physical map in IWGSC RefSeqV1.0 (International Wheat Genome Sequencing Consortium, 2018).