|
Antibodies
|
| in-house mAb 1C7 against SARS-CoV-2 NP protein, provided by Dr. Andrew Duty |
BioSS |
bs-41408P |
|
Bacterial and Virus Strains
|
| One Shot™ BL21(DE3) Chemically Competent E. coli
|
Invitrogen |
C600003 |
| Subcloning Efficiency™ DH5α Competent Cells |
Invitrogen |
18265017 |
| SARS-CoV-2 Victoria strain |
Tao Dong’s group, CAMS-Oxford Institute |
N/A |
| SARS-CoV-2 USA-WA1/2020 |
BEI Resources |
NR-52281 |
| SARS-CoV-2 Beta (B.1.351), Omicron (BA.5, XBB.1.5, XBB.1.16) |
Mount Sinai Pathogen Surveillance program |
IRB HS#13-00981 |
|
Biological Samples
|
|
|
|
| SARS-CoV-2 and variants, SARS-CoV pseudovirus particles (luciferase reporter) |
Integral Molecular |
RVP-702L, 707L, 770L, 774L, 786L, 789L, 792L, 794L, 801L |
|
Chemicals, Peptides, and Recombinant Proteins
|
| LB Miller Broth |
IBI Scientific |
49030 |
| Ampicillin, sodium salt, irradiated |
Gibco |
11593027 |
| LB Miller’s Agar Powder |
Growcells.com
|
MBPE-3020 |
| Dulbecco’s phosphate buffer saline no Ca 2+, Mg 2+ |
Thermo Fisher |
14190250 |
| Pierce™ Protease Inhibitor Tablets, EDTA-free |
Thermo Fisher |
A32965 |
| Tween® 20 |
Sigma |
P9416-100ml |
| Puromycin |
Gibco |
A1113803 |
| Penicillin-Streptomycin Solution, 100 x |
Corning |
30-002-CI |
|
Critical Commercial Assays
|
| Monarch® PCR & DNA Cleanup Kit |
NEB |
T1030L |
| Monarch® DNA Gel Extraction Kit |
NEB |
T1020S |
| EndoFree Plasmid Kits |
Qiagen |
12362 |
| ExpiFectamine 293 kit |
Thermo Fisher |
A14635 |
|
Renilla-Glo luciferase assay system |
Promega |
E2720 |
| Mycoplasma Detection Kit |
InvivoGen |
rep-mys-20 |
|
Deposited data
|
| Structure, simulation and open dynamics analysis |
Zenodo |
10.5281/zenodo.10684642 |
|
Experimental models: Cell lines
|
| Expi293F cells |
Thermo Fisher |
A14635 |
| Vero-E6 cell |
ATCC |
CRL-1586™
|
| Vero-E6-TMPRSS2 |
BPS Bioscience |
78081 |
| HeLa-ACE2 |
BPS Bioscience |
79958 |
| 293T-hsACE2 |
Integral Molecular |
C-HA101 |
|
Oligonucleotides
|
| ST3-F: 5’-cgcggatcccgtggcgtgccgcatattgtgatggtggatgcgtataaacggtacaagggtggaggtggctccatcgatac-3’ |
This paper |
N/A |
| ST3-R: 5’-gaggcagctcagcaat-3’ |
This paper |
N/A |
| NSC3-F: 5’-aagaatgcggccgcagggggccatattaaattctcaaaacgtgatgag-3’ |
This paper |
N/A |
| NSC3-R: 5’-ccgctcgagcccgggcccctggaacagaacttccagcccgccagtatgagcgtcacctt-3’ |
This paper |
N/A |
|
Recombinant DNA
|
| Human IgM-Fc |
Addgene (Li et al., 2020) |
158214 |
| Human IgM J-chain |
Addgene (Li et al., 2020) |
158215 |
| SpyCatcher003-mi3 |
Addgene (Rahikainen et al., 2021) |
159995 |
| Multiple Nbs and dimers in pET-21b(+) vector, multiple Nb-IgG-Fc in p3BNC vector |
Synbio Technologies |
N/A |
|
Software and Algorithms
|
| CryoSPARC |
(Punjani et al., 2017) |
https://cryosparc.com
|
| ChimeraX |
(Pettersen et al., 2021) |
https://www.cgl.ucsf.edu/chimerax/
|
| FreeSASA |
(Mitternacht, 2016) |
https://freesasa.github.io
|
| CHARMM-GUI webserver |
(Lee et al., 2016) |
https://www.charmm-gui.org
|
| MotionCor2 |
(Zheng et al., 2017) |
N/A |
| eTomo |
(Mastronarde and Held, 2017) |
N/A |
| IsoNet |
(Liu et al., 2022b) |
N/A |
|
Others
|
| HisPur™ Cobalt Resin |
Thermo fisher |
89966 |
| PROTEINDEXTM rProtein G Agarose 4 Fast Flow |
Marvelgent Biosciences, Inc. |
11-0206-025 |
