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. 2005 Jul 7;33(12):3733–3742. doi: 10.1093/nar/gki686

Figure 2.

Figure 2

Conversion of episomal eBFP to GFP. Representative dotplots of 293T cells 3 days after co-transfection with (A) pLacZ and 45-PS-eGFPas ON, (B) peBFP and 25-PS-PDEas ON and (C) peBFP and 45-PS-eGFPas ON as detailed in Materials and Methods. The control ON, 25-PS-PDEas, was of sequence complementary to βPDE. The control plasmid, pLacZ, was an expression vector for β-galactosidase. Blue fluorescent only cells are in the upper left quadrant (UL) and green and blue fluorescent cells, corresponding to converted cells, in the upper right (UR).