Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jul 7;33(12):3733–3742. doi: 10.1093/nar/gki686

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2005. Published by Oxford University Press. All rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org

PMC Copyright notice

Chromosomal eBFP conversion after transfection of 293T-eBFP cells with control or 25-LNA-eGFPas ONs. GFP expression was detected by fluorescence microscopy of 293T-eBFP cells 3 days after transfection with control (A) or 25-LNA-eGFPas ONs (C). Brightfield microscopy of 293T-eBFP cells transfected with control (B) or 25-LNA-eGFPas ONs (D). The control ON used was 25-LNA-PDEas of sequence complementary to photoreceptor cGMP-phosphodiesterase. BFP expression could be detected by western blot of 293T-eBFP cells but not by standard fluorescence microscopy due to its low fluorescence yield and strong photobleaching compared with GFP (14).