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. 2005 Jul 7;33(12):3733–3742. doi: 10.1093/nar/gki686

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© The Author 2005. Published by Oxford University Press. All rights reserved

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DNA sequence analysis of converted clones. Sequence of chromosomal eBFP target in GFP-positive clones of 293T-eBFP cells treated with 25-LNA-eGFPas. The coding sequence was amplified by PCR from genomic DNA and directly sequenced, which confirmed that the eBFP sequence had been converted to nGFP. The asterisk indicates the position of the modified base. The sequences shown, coding respectively Tyr⁶⁶ and Phe¹⁴⁵, were from the same chromatogram.