Figure 5.
Dose-dependent conversion of chromosomal eBFP by 25-LNA-eGFPas ON. (A) Rates of GFP-positive cells in 293T-eBFP cells at 3 days after transfection with indicated doses of 25-LNA-eGFPas ON. The amount of DNA in the transfection mixture was kept constant by the addition of a control LNA ON of unrelated sequence. (B) Rates of GFP-positive cells in 293T-eBFP cells transfected 1, 2 or 3 times with 25-LNA-eGFPas ON (determined 3 days after the last transfection). Results are expressed as means ± SD, represented as vertical bars, corresponding to triplicates from a single transfection experiment.