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DNA sequence analyses of corrected clones. (A) rd1 and wt-βPDE sequences. (B) Sequence of chromosomal rd1-eGFP* target in a GFP-positive clone of 293T-rd1-eGFP* cells treated 25-LNA-PDEas. The coding sequence was amplified by PCR from genomic DNA and directly sequenced, which confirmed that the rd1 sequence had been corrected into wt-βPDE. The asterisk indicates the position of the modified base.
