Figure 5.

GAGA-mediated enhancer recruitment to a promoter allows long distance activation in yeast. (A) Structure of the reporter system to monitor GAGA activity in yeast. The lacZ gene is under the control of the GAL1 promoter. Four Gal4p-binding sites (UAS_G) were inserted at the 3′ end of the lacZ gene. Upstream of the GAL1 promoter and adjacent to the (UAS_G) sites GAGA sites were introduced. (B) Expression of GAGA promotes transcriptional activation at large distances. The reporter construct depicted in (A) was integrated into the genome. The resulting reporter strain was transformed with Gal4p and/or GAGA factor expressing or empty plasmids. Expression of the lacZ reporter was quantified by β-galactosidase assays.