Figure 2.

(A) Assay result of enzyme activity of ADI_132–410 expressed with the fusion of FTN-H (FTN-H::ADI_132–410). (B) Result of SDS–PAGE analysis of the fusion protein, FTN-H::ppGRN in cell-free supernatant from whole-cell lysate of recombinant E.coli (lane 1), in the solution eluted by imidazole after loading the cell-free supernatant to Ni⁺² column for affinity purification of FTN-H::ppGRN (lane 2) and in the solution eluted after thrombin digestion to separate ppGRN from the Ni⁺²-bound FTN-H::ppGRN inside the Ni⁺² affinity column (lane 3).