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Cleavage at Arg-271 in the uncleaved zymogen. Prothrombin derivatives (5.8 μM) were digested with 1 nM prothrombinase (1 nM Xa/60 nM Va/50 μM PCPS) in the presence of 40 μM DAPA. The fraction of IIQ320 (○) and FPR-IIQ320 (•) remaining as a function of time was determined by SDS/PAGE and quantitative densitometry. The lines were arbitrarily drawn.
