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Summary of HTP N- and C-terminal cloning. (a) Gel showed N- and C-terminal fragments used for cloning and inserted fragments of clones. Symbols N and C indicate N- and C-terminal fragments, and I indicates insert fragments amplified by PCR. The dots indicate the position of missing fragments; green for N- or C-terminal; and yellow for right insert fragments. (b and c) Number of obtained colonies and the rate of right clone.
