Figure 6. Aurora kinase A inhibition in combination with anti-CTLA-4 treatment extends the survival of human papillomavirus-positive tumor-bearing mice. Groups of C57BL/6 mice were implanted with mEER cells (1×10⁶) subcutaneously on the flank and were treated sequentially with 10 mg/kg each of alisertib and anti-CTLA-4 antibody (aCTLA-4) individually or in combination (combo) (A) and monitored for tumor growth (B, C) and survival (D). Significant differences between the survival curves were determined using the log-rank (Mantel-Cox) test. ns, p≥0.05, *p<0.05, **p<0.005, ***p<0.0006, ****p<0.0001. CTLA-4, cytotoxic T-lymphocyte associated protein 4.