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. 2005 Jul 5;102(28):9936–9941. doi: 10.1073/pnas.0502552102

Fig. 3.

Fig. 3.

Microglia were the sole source for LPS-induced NO production. (A and B) Microglia expressed iNOS upon activation by LPS. preOLs and microglia cocultures were treated with LPS for 24 h. (A) Cocultures were evaluated for NADPH diaphorase activity. Arrows indicate preOLs, and arrowheads indicate NOS-positive microglia. (Scale bar, 20 μm.) (B) Cocultures were evaluated by immunocytochemistry for iNOS. Arrowhead indicates one microglial cell expressing iNOS and its marker ED1. (Scale bar, 10 μm.) (C) Nitrite production and Western blot analysis of preOLs and preOL plus microglia coculture treated with increasing concentrations of LPS. (D) Western blot analysis of iNOS of the indicated cell cultures treated with 1 μg/ml LPS in the presence or absence of l-NMMA for 24 h confirmed that only microglia (MG) challenged with LPS expressed iNOS. Data are representative of three independent experiments.