Fig. 6.
The microglial superoxide-generating NADPH oxidase contributed to LPS-induced toxicity to preOLs. (A and B) Microglia expressed the phagocytic NADPH oxidase. (A) Immunostaining of mixed glial cultures for gp91phox showed that only microglia stained strongly for gp91phox. (Scale bar, 25 μm.) (B) Western blot of different cultures assaying for components of NADPH oxidase. (C) Activation of NADPH oxidase by LPS. Western blot analysis showed translocation of p67phox to plasma membrane upon 1 μg/ml LPS challenge for 18 h. The same membrane was then reprobed for gp91phox.(D and E) NADPH oxidase inhibitor DPI (10 nM) prevented LPS-induced toxicity in preOL plus microglia cocultures. Data are representative of three separate experiments.