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. 2005 Jul 5;102(28):9936–9941. doi: 10.1073/pnas.0502552102

Fig. 7.

Fig. 7.

Microglia isolated from gp91phox-deficient mice failed to induce toxicity to preOLs. (A–C) Microglia deficient in gp91phox produced a similar level of NO when challenged with LPS. preOL plus microglia cocultures were treated with and without 1 μg/ml LPS for 24 h. (A) Western blot analysis. (B) Immunostaining of iNOS. (Scale bar, 10 μm.) (C) Nitrite levels (mean ± SD, n = 3). *, P < 0.05; **, P < 0.01; when compared with control; ns, not significant. (D and E) Microglia were isolated from gp91phox-deficient and wild-type mice and cocultured with preOLs. Cultures were treated with and without 1 or 5 μg/ml LPS for 24 h. (D) preOL survival. ***, P < 0.0001 compared with wild-type control; ##, P < 0.0001 compared with corresponding treatment for wild type. (E) Phase contrast image of cells treated with and without 5 μg/ml LPS. (Scale bar, 40 μm.) Results are representative of three independent experiments.