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. 2005 Jul 1;102(28):9872–9877. doi: 10.1073/pnas.0503961102

Fig. 4.

Fig. 4.

Reduced expansion of IL-4+ Th2 cells and B cells in the primary response of ICOS–/– mice to soluble BMA. (A) 4GET/ICOS+/– and 4GET/ICOS–/– mice were immunized in the footpad with 10 μg of BMA in CFA, and the popliteal lymph node cells were removed 10 days after immunization and analyzed by FACS. Representative FACS plot from two individual mice are shown. (B) Data compiled from three separate experiments showing the percentage of IL-4/GFP+CD4+ cells in the popliteal lymph node 10 days after immunization. (C) WT mice and ICOS–/– mice were immunized with CFA and PBS or CFA and BMA. The number of popliteal lymph node cells recovered 10 days after immunization is compiled from three separate experiments. (D) The cellular composition of the recovered popliteal lymph node cells was determined by FACS 10 days after immunization. The proportion of B cells is shown from two separate experiments.