Fig. 3.

Localization of MAGI-2, nephrin, IQGAP1, and αII spectrin in adult rat glomeruli. (A, C, and D) MAGI-2 (A), IQGAP1 (C), and αII spectrin (D) are distributed in a linear pattern along the glomerular capillaries. (B) Double labeling with nephrin shows that MAGI-2 and nephrin colocalize in the foot process layer of podocytes. Rat kidneys were cryoprotected, infiltrated with glucose, and processed for semithin cryosectioning. (A and B) Sections were fixed with acetone (3 min) before labeling for MAGI-2 and nephrin. (C and D) Rat kidneys were perfusion-fixed with PFA before processing for semithin cryosectioning. Sections were labeled with anti-IQGAP1 and anti-αII spectrin IgG and examined by immunofluorescence. (Bars: A–C, 15 μm; D, 7.5 μm.)