Table 4.

Primers for PCR

Gene assignment in strain NEM316	Gene assignment in strain 2603VR	Putative island	Primer Pairs
gbs0001	sag0001	-	5'-gtagctgatagtcctggc-3' and 5'-agtccccaactaaagcgc-3'
gbs0045-46	sag0046	MME	5'-aaatgggacacgtacgg-3' and 5'-attgccgccatctcaggg-3'
gbs0217 gbs0227	sag0224 sag0234	I	5'-caagcctttaatgctcgc-3' and 5'-aactgaaattccaatcgcc-3' 5'-tcatcgcgaaaatatggag-3' and 5'-cggtcttttagaaactgtgtcc-3'
gbs0247	sag0254	II	5'-gacttatttcaagtttatgg-3' and 5'-acccttatatacgacagc-3'
gbs0367 gbs0388 gbs0393	-	pNEM316-1 (islands III, VII and VIII)	5'-atcgatttaggattcatgcc-3' and 5'-caacattcgcaaaataagcc-3' 5'-cctagatggcgtagaggcag-3' and 5'-ttgctcacagaccataagcg-3' 5'-tcacccctgagacgtttacc-3' and 5'-gatcgtaaccacggtttgct-3'
gbs0467	sag0430	IV	5'-attgatagatcttacttgcg-3' and 5'-tgatgcaatagctattggc-3'
gbs0589 gbs0598	sag0610 sag0617	V	5'-cagggtgttcaaggctacc-3' and 5'-caagcttacgcacccaag-3' 5'-ctttcctaaaacatatttgg-3' and 5'-atatggtaaaaacttaaggc-3'
gbs0628 gbs0660	sag0645 sag0685	VI	5'-tagctcagtttgcgactgg-3' and 5'-ccaacttttgcatctgctg-3' 5'-aattcttgattgatgagcg-3' and 5'-tcagctttaatcaattccc-3'
-	sag0915	Tn916	5'-aagaccaaaagtggcgaac-3' and 5'-gcctttggattcattcctg-3'
gbs1050 gbs1073	sag1015 sag1038	IX	5'-agcagttacttgatttgcc-3' and 5'-tcctgaattagctagtcgc-3' 5'-tctgcttgagataactccc-3' and 5'-caatagcagttatcaaaggg-3'
gbs1120 gbs1125 gbs1135 gbs1143 gbs1145	-	X	5'-cctagatggcgtagaggcag-3' and 5'-ttgctcacagaccataagc-3' 5'-tcgacgtgttttacggttg-3' and 5'-accgaagagatgatgacgac-3' 5'-gggccacactagaaactgc-3' and 5'-aaatccttcatcgctcctg-3' 5'-tcacccctgagacgttacc-3' and 5'-gatcgtaaccacggtttgct-3' 5'-tctctcggcgttattgtcc-3' and 5'-acaaaagcacaagcgactg-3'
gbs1306 gbs1313	sag1233 sag1246	XII	5'-ctttactggcttcacttgg-3' and 5'-gttgatacaggcattgagc-3' 5'-gattactctaccagtgagg-3' and 5'-agaatagtctgcttcaccc-3'
gbs1987 gbs2008	sag2029 sag2053	XIII	5'-ctgacaattgctttgtttcg-3' and 5'-ggctaacccaaatgtaccg-3' 5'-gctcctctgattaatgccc-3' and 5'-caagctcttgttcggttgc-3'
gbs2082	sag2123	XIV	5'-tttctgggaaaaatcagtgg-3' and 5'-ttttcccgaacaaatgatg-3'