(A) Sequence alignment of Schizo. pombe Ulp1, Nep1 (amino acids 1–285) and Nep2 (amino acids 61–351) proteins with the human deneddylating enzyme NEDP1 created using CLUSTAL W. Black shading indicates identical residues and grey shading indicates conserved amino acids. (B) Comparison of the masses of the proteins relative to the position of the conserved cysteine residue (the asterisk indicates Cys163 in NEDP1). Ulp1, 568 amino acids; Nep1, 420 amino acids; Nep2, 415 amino acids; and NedP1, 212 amino acids.