Figure 4. Deletion of nep1 and nep2 does not affect the neddylation of cullins in vivo.

Total cell extracts were prepared from strains containing pcu1-myc (A), pcu3-myc (B) and pcu4-TAP (C) and analysed by Western blotting with anti-Myc antibodies (A, B), anti-mouse IgG (C) or anti-tubulin antibodies (as control). wt, wild type.