Table 1. Identification of 3-OST-1- and 6-OST-1-labelled oligosaccharides on bovine kidney HS.
The relationship between the m/z values of the monoisotopic peaks, the number of internal disaccharides (p), the number of NH2-containing disaccharides (n) and the number of sulphates (q) was calculated based on eqn (2), m/z=[340.09+379.11(p−n)+337.10n+79.96q−z]/z.
| Enzyme | Monoisotopic peak (m/z) | Elution time (min) | z | n | p | q | Formula |
|---|---|---|---|---|---|---|---|
| 3-OST-1 | 438.59 | 53.3 | 2 | 0 | 1 | 2 | dp4-1Ac-2S |
| 478.59 | 60.3 | 2 | 0 | 1 | 3 | dp4-1Ac-3S | |
| 6-OST-1 | 499.05 | 41.7 | 1 | 0 | 0 | 2 | dp2-2S(a)* |
| 499.05 | 44.2 | 1 | 0 | 0 | 2 | dp2-2S(b)* | |
| 708.19 | 55.0 | 1 | 0 | 0 | 3 | dp2-3S:1DBA* |
* Only major molecular species were listed.