Table 2. Calculation of the modification degrees (d) at 3-OST-1 and 6-OST-1 sites.
The intensities of isotopic peaks for 34S-saturated oligosaccharides by 3-OST-1 and 6-OST-1 were obtained through data acquisition. The initial intensities of the isotopic peaks were obtained by computer simulation. Peak intensities of monoisotopic peaks were arbitrarily set at 1 unit.
| m/z clusters | |||||||
|---|---|---|---|---|---|---|---|
| Enzymes | Oligosaccharide formula | Isotopic peak | m/z | Intensity | Initial intensity | ΔI3 | d (%) |
| 3-OST-1 | dp4-1Ac-2S | M | 438.59 | 1 | |||
| (C26H41O28N1S2) | M+2 | 439.59 | 6.12 | 0.20 | 5.92 | 14 | |
| dp4-1Ac-3S | M | 478.59 | 1 | ||||
| (C26H41O31N1S3) | M+2 | 479.58 | 27.86 | 0.25 | 27.61 | 4 | |
| 6-OST-1 | dp2-2S (a) | M | 499.05 | 1 | |||
| (C12H20O17S2) | M+2 | 501.05 | 0.80 | 0.13 | 0.67 | 60 | |
| dp2-2S (b) | M | 499.06 | 1 | ||||
| (C12H20O17S2) | M+2 | 501.06 | 0.71 | 0.13 | 0.58 | 63 | |
| dp2-3S:1DBA | M | 708.19 | 1 | ||||
| (C20H39O20N1S3) | M+2 | 710.20 | 1.17 | 0.21 | 0.96 | 51 | |