Fig. 4.

Pattern topography regulates macrophage morphology and phenotype in vitro. (A) Images and elongation factor of RAWs on PG hydrogels with different micropatterns. (B) Fluorescence images of RAWs on 10/20, 15/20, and flat gel immunostained for iNOS (M1 macrophage marker, green), Arg-1 (M2 macrophage marker, red), and nuclei [4′,6-diamidino-2-phenylindole (DAPI), blue]. Scale bars, 100 μm. (C) Expression of IL-6, iNOS, IL-10, and Arg-1 in RAWs cultured on hydrogel. Data are shown as fold change over glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (2^−ΔΔCt). Scale bars, 100 μm.