Fig. 5.

Pattern topography regulates fibroblast morphology and activation in vitro. (A) Images of RTFs on PG hydrogels after culturing for 72 h. (B) Immunofluorescence staining of F-actin. The illustration below shows the magnified cell morphology. (C) Statistical analysis of cell density and cell spreading area. (D) Optical density (O.D) values of RTFs on silicon, flat, 5-μm, 10-μm, 15-μm, and 20-μm pattern gels after 1, 3, and 5 d of culture. (E) Images of RTFs adhering to the pattern topography after culturing for 72 h with and (F) without profibrotic TGF-β. RTFs were stained for α-SMA (green) and nuclei (blue). (G) Statistical results of α-SMA relative expression. (H) Western blotting analysis of α-SMA expression of RTFs on flat hydrogel and 10/20 hydrogel before and after TGF-β stimulation. Scale bars, 100 μm.