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. 2005 Jul 5;389(Pt 2):577–585. doi: 10.1042/BJ20050159

Figure 5. Immunofluorescence labelling of ABCA17 protein in ABCA17-transfected cells and mouse testis.

Figure 5

(A–D) Confocal microscopic images of HEK293 cells expressing ABCA17–HA fusion protein. Cells were double-labelled with anti-HA antibody (A; green) and anti-ABCA17 antibody (B; red), and visualized with FITC and Cy3 respectively. Nuclei were counterstained by DAPI (C; blue). A merged picture of (A), (B) and (C) is shown in (D). (E–H) Confocal microscopic images of HEK293 cells co-expressing ABCA17–HA fusion protein and DsRed2-ER. ABCA17–HA fusion protein immunolabelled with anti-HA antibody was visualized with FITC (E; green), and the ER was observed with DsRed2 fluorescence (F; red). Nuclei were counterstained by DAPI (G; blue). A merged picture of (E), (F) and (G) is shown in (H). (I–L) Confocal microscopic images of HEK293 cells co-expressing ABCA17–HA fusion protein and DsRed2-Mito. ABCA17–HA fusion protein immunolabelled with anti-HA antibody was visualized with FITC (I; green) and mitochondria were observed with DsRed2 fluorescence (J; red). Nuclei were counterstained by DAPI (K; blue). A merged picture of (I), (J) and (K) is shown in (L). (M–O) Confocal microscopic images of mouse testis. A frozen mouse testis section immunolabelled with anti-ABCA17 antibody was visualized with Cy3 (M; red). Nuclei were counterstained by DAPI (N; blue). A merged picture of (M) and (N), and its magnified three-dimensional image, are shown in (O) and (P) respectively. The scale bar represents 5 μm (A–L), 50 μm (M–O) and 10 μm (P).