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. 2001 Dec 26;99(1):66–71. doi: 10.1073/pnas.012606199

Figure 1.

Figure 1

Linearity of signals for mouse hypothalamic mRNAs in RNase protection analysis. (A) A mixture of 32P-labeled cRNA probes for mouse AgRP, CART, NPY, and POMC was hybridized to total hypothalamic RNA (0, 5, 10, 15 μg) for 10 min at 68°C. The position of migration of each protected fragment is indicated: lane A, AgRP (396 bp); lane C, CART (159 bp); lane N, NPY (205 bp); lane P, POMC (304 bp); lane Cy, cyclophilin (103 bp). Exposure of the phosphorimaging plate was 24 h at room temperature. (B) Results from A quantified by phosphorimager. (a) AgRP; (b) CART; (c) NPY; (d) POMC; (e) cyclophilin. MWM, molecular weight markers. PSL, photostimulated luminescence.