Table 2.

Broad-Substrate Screen for acetyltransferase function using AcCoA as a donor.

Acceptor substrate	SidF variant	1Activity (µmol/min*mg)	p-values
			2Own background	3SidF WT
Cadaverine	SidF WT	0.650 (0.745)	0.123	1.644E-07
L-Lysine		−0.008 (0.010)	0.148	5.447E-07
L-Glutamic acid		−0.017 (0.024)	0.186	5.360E-07
L-Ornithine		−0.018 (0.015)	0.050	5.381E-07
Glycine		0.013 (0.107)	0.797	4.821E-07
Kanamycin A		0.003 (0.140)	0.970	4.303E-07
Ampicilin		0.025 (0.039)	0.221	5.549E-07
Chloramphenicol		0.012 (0.043)	0.561	5.445E-07
NAD		−0.035 (0.032)	0.070	5.231E-07
Urea		−0.015 (0.040)	0.459	5.304E-07
Guanidine		−0.005 (0.013)	0.395	5.460E-07
GSSH		−0.024 (0.028)	0.130	5.310E-07
Hydroxylamine		0.476 (0.235)	0.011	5.084E-07
N(5)-Hydroxy-L-ornithine		7.03 (1.47)	5.504E-07	−
N(5)-Hydroxy-L-ornithine	SidF N-ter	−0.014 (0.054)	0.660	5.371E-07
N(5)-Hydroxy-L-ornithine	SidF C-trim	2.15 (0.613)	0.001	2.123E-06

Note¹Standard deviation; ²One-Sample t-Test; ³Welch's t-test (two-sample t-test with unequal variances); NAD: Nicotinamide Adenine Dinucleotide; GSSH: Oxidized Glutathione. Data points represent the mean of n = 5 replicates, except for SidF WT tested with N(5)-Hydroxy-L-ornithine, where n = 9.