Table 2.
Top 10 up/down-regulated characterized genes in EPA-treated C. albicans biofilms identified by RNA-seq.
| Gene name | Log2 (fold change) a | Description b |
|---|---|---|
| Up-regulated | ||
| TNA1 | 4.67 | Putative nicotinic acid transporter; detected at germ tube plasma membrane by mass spectrometry; rat catheter biofilm induced |
| EBP1 | 3.53 | NADPH oxidoreductase; possible role in estrogen response; induced by oxidative, weak acid stress, NO, benomyl, GlcNAc; Cap 1, Mnl1 induced; Hap43-repressed; rat catheter biofilm induced |
| FGR41 | 3.33 | Putative GPI-anchored adhesin-like protein involved in the regulation of covering cell wall glucan; transposon mutation affects filamentous growth; Spider biofilm repressed |
| ACE2 | 3.14 | Transcription factor; regulates morphogenesis, cell separation, adherence, and virulence in mice; mutant is hyperfilamentous; rat catheter and Spider biofilm induced |
| JEN2 | 3.03 | Dicarboxylic acid transporter; regulated by glucose repression; induced by Rgt1; rat catheter and Spider biofilm induced |
| SCW11 | 2.75 | Cell wall protein; repressed in ace2 mutant; repressed in core caspofungin response; induced in high iron; possibly an essential gene; rat catheter and Spider biofilm repressed |
| OYE22 | 2.69 | Putative NADPH dehydrogenase; rat catheter biofilm induced |
| CHT 3 | 2.63 | Major chitinase; secreted; putative signal peptide; hyphal-repressed; farnesol upregulated in biofilm; regulated by Efg1p, Cyr1p, Ras1p |
| HSP31 | 2.63 | Putative 30 kDa heat shock protein; repressed during the mating process; rat catheter biofilm induced |
| HHT21 | 2.59 | Putative histone H3; amphotericin B repressed; regulated by Efg1, farnesol; Hap43-induced; rat catheter and Spider biofilm repressed |
| Down-regulated | ||
| CFL11 | −4.44 | Superoxide-generating NADPH oxidase produces an extracellular burst of reactive oxygen species at growing cell tips during hyphal morphogenesis; regulated by Cdc42p |
| PRA1 | −4.39 | Cell surface protein that sequesters zinc from host tissue; enriched at hyphal tips; released extracellularly; binds to host complement regulators |
| BTA1 | −3.65 | Betaine lipid synthase |
| ZRT2 | −3.35 | Zinc transporter, essential for zinc uptake and acidic conditions tolerance; induced in oralpharyngeal candidiasis; Spider biofilm induced |
| HGT9 | −3.34 | Putative glucose transporter of the major facilitator superfamily; the C. albicans glucose transporter family comprises 20 members; 12 probable membrane-spanning segments |
| GIT1 | −3.22 | Glycerophosphoinositol permease; involved in utilization of glycerophosphoinositol as a phosphate source; Rim101-repressed; virulence-group-correlated expression |
| PGA50 | −3.16 | Putative GPI-anchored protein; adhesin-like protein |
| CWH8 | −3.02 | Putative dolichyl pyrophosphate phosphatase; ketoconazole-induced; expression is increased in a fluconazole-resistant isolate; Hap43p-induced gene |
| SSU1 | −3.01 | Protein similar to S. cerevisiae Ssu1 sulfite transport protein; Tn mutation affects filamentous growth; regulated by Gcn2 and Gcn4; Hap43-repressed; Spider and flow model biofilm induced |
| FGR2 | −2.94 | Protein similar to phosphate transporters; transposon mutation affects filamentous growth; expression is regulated upon white-opaque switching |
a
The Log2 (fold change) was derived from RNA-seq results with an FDR of 0.05.
b
As reported in the CGD database (http://www.candidagenome.org/).