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. 2001 Dec 18;99(1):90–94. doi: 10.1073/pnas.221596698

Figure 1.

Figure 1

Purification of a Set1p-containing protein complex from S. cerevisiae. (A) A crude extract of S. cerevisiae proteins was passed through a gel filtration column, and the resulting fractions were analyzed by Western blot analysis using an anti-Set1p monoclonal antibody or mouse antisera specific for Set1p complex components Bre2p, Saf41p, Saf37p, and Saf19p (indicated to the right of respective panels). (B) Silver-stained SDS 4–16% gradient PAGE of gel-filtration fractions of immunopurified Set1p complex after propylene glycol-assisted release from anti-Set1p affinity column. Dots indicate complex components from top to bottom: Set1p, Bre2p, Saf49p, Saf41p, Saf37p, Saf35p, and Saf19p, the products of the SET1 gene and ORFs YLR015W, YAR003W, YPL138C, YKL018W, YBR175W, and YDR469W, respectively.