Table 2.
Representative B2 mutant sequences
| Sequence name | % Plasmid loss/generation | Sequence | Best match to ACS/11 | Helical stability (Kcal/mol) | No. of As/11 | No. of Ts/11 |
|---|---|---|---|---|---|---|
| WILDTYPE | 3.1 ± 1.1 | AATACTTAAAT | 9 | 2.8 | 6 | 4 |
| B2-802-10 | 7.8 ± 1.0 | CTCGAGGAAAT | 6 | 8.1 | 4 | 2 |
| B2-M1 | 2.4 ± 2.1 | AAACAAATATA | 8 | 3.4 | 8 | 2 |
| B2-M2 | 2.9 ± 0.4 | AAAATGATATA | 8 | 3.16 | 7 | 3 |
| B2-M3 | 3.0 ± 1.9 | AATTACGAAAT | 8 | 4.5 | 6 | 3 |
| B2-M4 | 4.6 ± 1.3 | ATAAACCGCCA | 7 | 8.7 | 5 | 1 |
| B2-M5 | 7.1 ± 2.2 | CTTGGCTCGCC | 3 | 12.3 | 0 | 3 |
| B2-M6 | 7.5 ± 0.6 | TTTCGTACCGC | 4 | 9.6 | 1 | 4 |
| B2-M7 | 10.4 ± 0.6 | TCTTTTAGCAT | 4 | 5.3 | 2 | 6 |
| B2-M8 | 10.7 ± 1.0 | CCAAGTTTCTT | 4 | 7.2 | 2 | 5 |
| B2-M9 | 15.0 ± 6.6 | CTTCCTGGCTT | 4 | 9.4 | 0 | 5 |
| B2-M10 | 23.1 ± 6.4 | TGATCCTCACA | 6 | 8.2 | 3 | 3 |
Plasmid loss assays were performed at least three times. Best match to the ACS and helical stability were calculated as in Table 1.