Skip to main content
. Author manuscript; available in PMC: 2025 Jan 22.
Published in final edited form as: Gastroenterology. 2020 Jun 20;159(4):1375–1389. doi: 10.1053/j.gastro.2020.06.038

Figure 4.

Figure 4.

The effect of anti-miR-200c-3p transfection and miR-200c-3p overexpression on mouse small intestinal permeability in vivo. Effect of anti-miR-200c-3p transfection on IL-1β induced increase in mouse enterocyte miR-200c-3p expression (A). Mouse intestinal mucosal surface was transfected anti-miR-200c-3p in vivo. Effect of anti-miR-200c-3p transfection on IL-1β induced decrease in mouse enterocyte occludin mRNA expression (B), and increase in mouse intestinal permeability(C). Mouse small intestinal mucosal surface were transfected with pre-miR-200c-3p in vivo. Enterocytes were isolated from the mucosal surface by LCM. Effect of pre-miR-200c-3p transfection on mouse enterocyte miR-200c-3p (D), and occludin mRNA (E) expression. Effect of pre-miR-200c-3p and anti-miR-200c-3p transfection on intestinal tissue occludin protein expression (F), and pre-miR-200c-3p transfection on mouse intestinal permeability in vivo (G). * P < .01 vs control, * * P < .01 vs IL-1β treatment.