Figure 2.

Dose-dependent inhibition of the enzymatic activity of MIF by acetaminophen (3) and its metabolites (4–6). MIF (60 nM) was incubated with test compounds for 5 min at room temperature, and the tautomerase activity was measured by using dopachrome methyl ester (1) as a substrate (25). Recombinant human MIF was expressed in E. coli and purified as described (35).