Figure 1.
UV-induced translocation of CSA protein to the nuclear matrix in dtCSA/CS3BESV cells. Asynchronous cells were irradiated with 20 J/m2 of UV or nonirradiated and incubated for the indicated period, and then the cellular extracts were prepared as described in Materials and Methods. Proteins from each fraction with equivalent cell numbers were loaded for SDS/PAGE and analyzed by immunoblotting with anti-HA antibody. The lane numbers correspond to the fraction numbers.