Figure 5.
Translocation of CSA protein to the nuclear matrix by chemical agents whose damage is subjected to TCR. (A) The dtCSA/CS3BESV cells were irradiated with 20 J/m2 of UV and incubated for 60 min or treated (incubated) with DNA damaging agents (100 μM cisplatin; 10 mM hydrogen peroxide; 150 μM DMS). Each fraction was prepared from cells and then immunoblotted with anti-HA antibody. The lane numbers correspond to the fraction numbers. (B) XP-A (XP2OSSV), XP-C (XP4PASV), CS-B (CS1ANSV), wild-type (WI38VA13) cells, and CS1ANSV cells expressing the dtCSB construct (dtCSB/CS1ANSV) were treated with 10 mM hydrogen peroxide and then immunoblotted with anti-CSA antibody. The lane numbers correspond to the fraction numbers.