REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
septin7	Abcam	Cat#ab17522; RRID: AB_2876836
PIK3CA	Cell Signaling Technology	Cat#4249S; RRID: AB_2165248
Orai	ABclonal	Cat#A7412; RRID: AB_2767942
IP3R	Abways	Cat# Q14643; RRID: AB_3086675
AKT	Cell Signaling Technology	Cat#4691S; RRID: AB_915783
Caspase-3	Cell Signaling Technology	Cat#14420T; RRID: AB_2798429
cleaved-caspase-3	Cell Signaling Technology	Cat#9664; RRID: AB_2070042
Tubulin	Proteintech	Cat#66301-1-Ig; RRID: AB_11042766
β-actin	Yeasen	Cat#30103ES; RRID: AB_2923152
Secondary antibodies HRP-linked anti-rabbit IgG antibody	Cell Signaling Technology	Cat#7074; RRID: AB_2099233
HRP-linked anti-mouse IgG antibody	Cell Signaling Technology	Cat#7076S; RRID: AB_330924
Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555	Thermo Fisher Scientific	Cat#A32727; RRID: AB_2633276
FLAG Tag Antibody (magnetic bead conjugate)	Cell Signaling Technology	Cat#82103S; RRID:AB_3073821
Myc-Tag Antibody (magnetic bead conjugate)	Cell Signaling Technology	Cat#91856S; RRID: AB_3073821
control IgG (magnetic bead conjugate)	Cell Signaling Technology	Cat#8726S; RRID: AB_10828938
Chemicals, peptides, and recombinant proteins
Polybrene	Sigma-Aldrich	Cat# 107689
Puromycin	Thermo Fisher Scientific	Cat# A1113803
RIPA lysate	Yeasen	Cat#20114ES60
PMSF	Yeasen	Cat#20104ES03
septin7 protein	Abcam	Cat#ab158072
cocktail protease inhibitors	Abcam	Cat#ab158072
DAPI	Coolabor	Cat# SL1086
CCK-8	Beyotime	Cat# C1002
Crystal Violet Staining Solution	TargetMol	Cat#C0005
TRIzol reagent	Beyotime	Cat# C1021
Critical commercial assays
BCA Protein Quantification Kit	Yeasen	Cat#20201ES76
Annexin V-FITC Apoptosis Detection Kit	Elabscience	Cat#E-CK-A211
Cell Cycle and Apoptosis Analysis Kit	Beyotime	Cat#C1052
Total RNA Kit II	Omega	Cat#R6934-01
Hifair II 1st Strand cDNA Synthesis SuperMix	Yeasen	Cat# 11123ES60
Hieff qPCR SYBR® Green Master Mix	Yeasen	Cat#11201ES08
Capturem Streptavidin Miniprep Columns Kit	Takara	Cat#635733
Fast Silver Stain Kit	Beyotime	Cat#P0017S
Fluo-4 a.m. (Calcium ion fluorescent probe, 2mM)	Beyotime	Cat#S1060
Deposited data
RNA-Seq data	This paper	GSE236228
Experimental models: Cell lines
ISK	ATCC	HTB-113
KLE	ATCC	CRL-1622
Experimental models: Organisms/strains
Mouse: BALB/c nude	Shanghai SLAC Laboratory Animal Co., Ltd	https://www.bioon.com.cn/company/index/c42d1349852
Oligonucleotides
shRNA targeting sequences (see Table S7)	This paper	N/A
Primers for RT-qPCR (see Table S8)	This paper	N/A
Software and algorithms
GraphPad Prism version 8.0	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
ImageJ	ImageJ software	https://imagej.net/ij/download.html
FlowJo v10.8.1	FlowJo software	https://www.flowjo.com/solutions/flowjo
Real-time PCR	Bio-Rad	CFX384
CytExpert2.4	Beckman Coulter Life Sciences	https://www.beckman.ae/flowcytometry/instruments/cytoflex/software