Figure 3.

Pyrrocidines A and B act synergistically to inhibit F. verticillioides WT growth. Plot of the fitted model values illustrating the mean reduction in F. verticillioides WT growth from 10 μg/mL pyrrocidine A alone (pink) or 10 μg/mL pyrrocidine B alone (green). The expected additive effect (brown) describes the expected, calculated inhibition of 20 μg/mL pyrrocidine A + B if pyrrocidine A and B acted additively to reduce growth. This expected additive value is the sum of two differences: the difference between the DMSO control mean and the 10 μg/mL pyrrocidine A mean; plus, the difference between the DMSO control mean and the 10 μg/mL pyrrocidine B mean. The observed synergistic value (yellow) represents the observed reduction in growth from 20 μg/mL pyrrocidine A + B, which is significantly higher than the expected additive value for each growth parameter [total growth (area under the optical density (OD) growth curve), maximum OD growth rate, and final OD (OD at the final time point)]. This means that pyrrocidines A and B act synergistically—not additively—to inhibit F. verticillioides WT growth. Medians of the posterior estimates of the growth parameters are shown as points and credible intervals as progressively lighter-shaded bars. CI, credible interval.