Figure 5.

F. verticillioides ΔFvZBD1 mutant fumonisin biosynthesis was generally not inhibited by pyrrocidine challenge. The F. verticillioides ΔFvZBD1 mutant was challenged with the following treatment conditions: 0.5% DMSO control; 0.5% DMSO containing either pyrrocidine A, B, or combination A + B at the following final concentrations, (1) 0.5, (2) 1.0, (3) 2.5, (4) 5.0, (5) 10, and (6) 20 μg/mL, and monitored for 120 h, at 28°C, with continuous shaking in the dark in modified Fusarium minimal media. Plots represent ΔFvZBD1 fumonisin production in all treatment conditions, normalized to growth [optical density (OD600)]. Data was collected at 120 h, following the conclusion of the pyrrocidine dose-response assay. Data from a single experimental replicate is presented. The graph shows the distribution of the raw data (as box and whisker plots) and the means with 95% confidence intervals for fumonisin type (FB₁, FB₂, or FB₃) and concentration per treatment. The multiple comparison letters, for each combination of fumonisin and pyrrocidine, are valid within each panel.