Mammary Epithelial Cell Growth Media (MEGM)

Reagent	Final concentration	Amount
DMEM/F-12	N/A	500 mL
Insulin (10 mg/mL)	5 μg/mL	250 μL
Hydrocortisone (1 mg/mL)	500 ng/mL	250 μL
EGF (100 μg/mL)	10 ng/mL	50 μL
Cholera toxin (200 μg/mL)	20 ng/mL	50 μL
FBS	5%	25 mL
Gentamicin (50 mg/mL)	50 μg/mL	500 μL
Total	N/A	526.1 mL

Filter with 0.2 μm bottle top filtration device. Store at 4°C for up to 1 month.