Dissection medium

Reagent	Final concentration	Amount
DMEM/F12 (+HEPES, no phenol red)	87%	43.5 mL
Heat-inactivated FBS (see above)	10%	5 mL
Penicillin/streptomycin 100×	1×	0.5 mL
ITS-X 100×	1×	0.5 mL
GlutaMAX 100×	1×	0.5 mL
APE 2000×	1×	25 μL
Total	–	50 mL

Store at +4°C for up to 1 month.