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. 2024 Dec 10;66(1):100726. doi: 10.1016/j.jlr.2024.100726

Table 1.

Summary of studies assessing ceramide levels in patients with cardiometabolic diseases

Disease Participants Ceramide Type of sample Refs
IR Individuals with BMI <27 kg/m2 were classified as lean control subjects (n = 10). Subjects with a BMI of 27–33 kg/m2 were classified as obese without diabetes (n = 10) The total muscle ceramide content was nearly 2-fold (46 ± 9 vs. 25 ± 2 pmol/2 mg muscle, P < 0.05) Human muscle tissue (16)
IR risk Male subjects between 20 and 40 years of age (n = 27) There was a significant negative correlation between muscle total ceramide content and insulin sensitivity (r = −0.49, P = 0.01) Human muscle tissue (64)
T2D FINRISK 2002 (n = 8,045), Western Norway Coronary Angiography Cohort (n = 3,344) Ceramide ratios: C18:0/C16:0 ceramide increased Human plasma (19)
T2D Obese sedentary controls (n = 14), T2D patients (n = 15), and endurance training athletes (n = 15) C18:0, C20:0, and C24:1 ceramides increased Human serum (65)
T2D T2D patients (n = 13), healthy nondiabetic control subjects (n = 14) C18:0, C20:0, C24:1 ceramides increased Human serum (54)
T2D Cardiovascular health study participants (n = 3,645) C16:0, C18:0, C20:0, C24:1 ceramides increased Human serum (55)
T2D Strong Heart Study (n = 435) and Strong Heart Family Study (n = 1902) participants C18:0, C20:0, C22:0 ceramides increased Human serum (56)
T2D, CVD Strong Heart Study (n = 267) and Strong Heart Family Study (n = 597) participants Higher plasma C16:0 ceramide levels in patients with T2D are associated with a higher risk of CVD Human blood (57)
T2D T2D patients (n = 335) There was an independent association between liver fat content and plasma levels of C18:0, C20:0, C22:0, and C24:0 ceramides Human plasma (58)
CAD Familial CAD (n = 462) and population-based controls (n = 212) C18:0, C22:0, C24:0 ceramides increased Human serum (59)
CAD CAD (n = 265) and no CAD (n = 230) C16:0, C18:0, C24:1 ceramides increased Human plasma (18)
CAD Subjects in the Corogene (n = 160), Special Program University Medicine—Inflammation in Acute Coronary Syndromes (n = 1,637), and Bergen Coronary Angiography Cohort studies (n = 81) Ceramide ratios: C16:0/C24:0, C18:0/C24:0, C24:1/C24:0 ceramide increased Human plasma (60)
CHD, HF Framingham Heart Study (n = 2,642) and study of Health in Pomerania (n = 3,134) participants C24:0/C16:0 ceramide ratios were inversely correlated with coronary heart disease events, and C24:0/C16:0 and C22:0/C16:0 ceramide ratios were negatively correlated with all-cause mortality. Human serum (61)
Heart failure (HF) Cardiovascular Health Study participants (n = 4,249) Higher levels of C16:0 ceramide in plasma are associated with an increased risk of HF, whereas higher levels of C22:0 ceramide are associated with a reduced risk of HF Human serum (62)
Hypertensive patients at high CV risk Hypertensive patients aged 18–85 years with high or very high cardiovascular risk (n = 225) C16:0, C22:0, and C24:0 ceramides have a significant significance in predicting major adverse cardiovascular event Human blood (63)
NAFLD Obese children (n = 80), including children with NAFLD (n = 31) C14:0, C16:0, C16:1, C18:0, C18:1, C22:0, C24:0 ceramides increased Human serum (66)
NAFLD Individuals with liver histological classification of normal (n = 31), steatosis (n = 17), NASH (n = 20), or cirrhosis (n = 20) Plasma: C18:0, C18:1, C20:0, C22:0, C24:0, C24:1 ceramides increased; liver:C18:0, C20:0, C22:0, C24:0 ceramides increased Human liver and plasma (67)
NASH Morbidly obese women (n = 46), of which NASH patients (n = 22) Ceramides increased Human serum and portal vein blood (68)
IR, NAFLD HOMA-IR >3.19 (n = 62), HOMA-IR ≤3.19 (n = 63) C16:0, C18:0, C19:0, C20:0, C24:1 ceramides increased Human liver (14)
NASH Obese (BMI >30 kg/m2) patients (n = 21) and 7 control subjects. Liver histology classifies obese patients into NAFL (n = 7), NAFL+ (n = 7), and NASH (n = 7) Liver: ceramides, dihydroceramide, and lactosylceramides increased; serum: dihydroceramide increased Human liver and serum (69)

HF, heart failure; HOMA-IR, homeostasis model assessment of IR; NAFL, obese patients without steatosis; NAFL+, obese patients with steatosis; NAFLD, nonalcoholic fatty liver disease; NASH, nonalcoholic steatohepatitis.