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HPLC elution profile of an extract of eggs or stage 1 embryos. (A) The chromatographic profile exhibits a number of peaks with distinctive characteristics. The peak pertinent to this report elutes with a retention time of 23.3 min (arrow). (B) Comparison of peak areas of the HPLC profiles of extracts from control (black) and 366-nm UV light irradiated embryos (gray) demonstrate that the 23.3-min peak is the only one that is markedly reduced.
