Table 3.

Multiplex PCR evaluation with representative possible genotypes.

Genotype	Multiplex Mix A—gene/allele (bp)					Multiplex Mix B—gene/allele (bp)					Predicted Phenotype
	HGH	GYPA	GYPA*M, c.71T and c.72T	GYPA*M, c.59 C	GYPB*S, c.143T	HGH	GYPA	GYPA*N, c.71 A and c.72G	GYPA*N, c.59 C	GYPB*s, c.143 C
	(1,070)	(538)	(318)	(273)	(118)	(1,070)	(538)	(318)	(273)	(118)
GYPA*M/M, GYPB*S/S	+	+	+	+	+	+	+	–	–	–	M + N– S + s–
GYPA*M/M, GYPB*S/s	+	+	+	+	+	+	+	–	–	+	M + N– S + s+
GYPA*M/M, GYPB*s/s	+	+	+	+	–	+	+	–	–	+	M + N– S– s+
GYPA*M/N, GYPB*S/S	+	+	+	+	+	+	+	+	+	–	M + N + S + s–
GYPA*M/N, GYPB*S/s	+	+	+	+	+	+	+	+	+	+	M + N + S + s+
GYPA*M/N, GYPB*s/s	+	+	+	+	–	+	+	+	+	+	M + N + S– s+
GYPA*N/N, GYPB*S/S	+	+	–	–	+	+	+	+	+	–	M– N + S + s–
GYPA*N/N, GYPB*S/s	+	+	–	–	+	+	+	+	+	+	M– N + S + s+
GYPA*N/N, GYPB*s/s	+	+	–	–	–	+	+	+	+	+	M– N + S– s+

Bp base pair, GYPA Glycophorins A, GYPB Glycophorins B, HGH Human growth hormone.